{"id":1164,"date":"2022-09-17T17:03:19","date_gmt":"2022-09-17T16:03:19","guid":{"rendered":"https:\/\/rdu.if.ua\/?p=1164"},"modified":"2022-09-17T17:03:19","modified_gmt":"2022-09-17T16:03:19","slug":"high-fat-high-fructose-diet-induces-mild-oxidative-stress-and-reorganizes-intermediary-metabolism-in-male-mouse-liver-alpha-ketoglutarate-effects","status":"publish","type":"post","link":"https:\/\/rdu.if.ua\/en\/high-fat-high-fructose-diet-induces-mild-oxidative-stress-and-reorganizes-intermediary-metabolism-in-male-mouse-liver-alpha-ketoglutarate-effects\/","title":{"rendered":"High fat high fructose diet induces mild oxidative stress and reorganizes intermediary metabolism in male mouse liver: Alpha-ketoglutarate effects"},"content":{"rendered":"[vc_row type=”in_container” full_screen_row_position=”middle” column_margin=”default” column_direction=”default” column_direction_tablet=”default” column_direction_phone=”default” scene_position=”center” text_color=”dark” text_align=”left” row_border_radius=”none” row_border_radius_applies=”bg” overflow=”visible” overlay_strength=”0.3″ gradient_direction=”left_to_right” shape_divider_position=”bottom” bg_image_animation=”none”][vc_column column_padding=”no-extra-padding” column_padding_tablet=”inherit” column_padding_phone=”inherit” column_padding_position=”all” column_element_spacing=”default” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” column_link_target=”_self” column_position=”default” gradient_direction=”left_to_right” overlay_strength=”0.3″ width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” animation_type=”default” bg_image_animation=”none” border_type=”simple” column_border_width=”none” column_border_style=”solid”][vc_column_text css=”.vc_custom_1663430541452{padding-bottom: 3% !important;}”]\"\"
\nDiets rich in fats and\/or carbohydrates are used to study obesity and related metabolic complications. We studied the effects of a high fat high fructose diet (HFFD) on intermediary metabolism and the development of\u00a0oxidative stress<\/a> in mouse liver and tested the ability of alpha-ketoglutarate to prevent HFFD-induced changes.
\nMale mice were fed a standard diet (10% kcal fat) or HFFD (45% kcal fat, 15% kcal fructose) with or without addition of 1% alpha-ketoglutarate (AKG) in drinking water for 8\u00a0weeks.
\nThe HFFD had no effect on body mass but activated fructolysis and\u00a0glycolysis<\/a>\u00a0and induced inflammation and oxidative stress with a concomitant increase in activity of antioxidant enzymes in the mouse liver. HFFD-fed mice also showed lower mRNA levels of\u00a0pyruvate dehydrogenase kinase<\/a>\u00a04 (PDK4) and slightly increased intensity of\u00a0mitochondrial respiration<\/a>\u00a0in liver compared to mice on the standard diet. No significant effects of HFFD on transcription of\u00a0PDK2<\/a><\/em>\u00a0and\u00a0PGC1\u03b1<\/em>, a peroxisome proliferator-activated receptor co-activator-1\u03b1, or protein levels of p-AMPK, an active form of AMP-activated protein kinase, were found. The addition of AKG to HFFD decreased oxidized\u00a0glutathione<\/a>\u00a0levels, did not affect levels of\u00a0lipid peroxides<\/a>\u00a0and\u00a0PDK4<\/em>\u00a0transcripts but increased activities of\u00a0hexokinase<\/a>\u00a0and\u00a0phosphofructokinase<\/a>\u00a0in mouse liver.
\n<\/span>[\/vc_column_text][nectar_btn size=”large” open_new_tab=”true” button_style=”see-through” button_color_2=”Accent-Color” color_override=”#2954aa” icon_family=”default_arrow” text=”Read the full article” url=”https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0304416522001441″][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

Diets rich in fats and\/or carbohydrates are used to study obesity and related metabolic complications. We studied the effects of a high fat high fructose diet (HFFD) on intermediary metabolism and the development of oxidative stress in mouse liver and tested the ability of alpha-ketoglutarate to prevent HFFD-induced changes.<\/p>\n","protected":false},"author":1,"featured_media":1170,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"footnotes":""},"categories":[17],"tags":[],"class_list":{"0":"post-1164","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-researches-en"},"_links":{"self":[{"href":"https:\/\/rdu.if.ua\/en\/wp-json\/wp\/v2\/posts\/1164","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/rdu.if.ua\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/rdu.if.ua\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/rdu.if.ua\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/rdu.if.ua\/en\/wp-json\/wp\/v2\/comments?post=1164"}],"version-history":[{"count":1,"href":"https:\/\/rdu.if.ua\/en\/wp-json\/wp\/v2\/posts\/1164\/revisions"}],"predecessor-version":[{"id":1172,"href":"https:\/\/rdu.if.ua\/en\/wp-json\/wp\/v2\/posts\/1164\/revisions\/1172"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/rdu.if.ua\/en\/wp-json\/wp\/v2\/media\/1170"}],"wp:attachment":[{"href":"https:\/\/rdu.if.ua\/en\/wp-json\/wp\/v2\/media?parent=1164"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/rdu.if.ua\/en\/wp-json\/wp\/v2\/categories?post=1164"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/rdu.if.ua\/en\/wp-json\/wp\/v2\/tags?post=1164"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}